Should I put them back together, or should I be good?
If she is showing gravid colors you should be good. Gravid colors being black and pink
I have seen timeframes mentioned for anywhere from 10 days to 2 months for the gestation period. What should I be expecting realistically?
22-34 days
Wondering when I should put laying medium with her.
You can put it in now It will give her a chance to get use to it being in her cage.
That brings up anothern question. There are many many opinions for the laying medium, depth of the medium, how much water it should have, what is best?
For laying bin use a container, bucket or trash can that is at least 9 inches deep. Fill it with soil or sand or a soil sand mixture. I use topsoil found at walmart or home depot. Just be sure whatever you get does not have fertilizer or any chemicals in it. You want the soil to be moist but not soaking wet. You want to be able to dig a tunnel in the dirt without it collapsing. If the bin is in her cage for a few weeks be sure to keep it clean and remove any poo that might fall into the soil.
When the eggs are laid should I remove them?
When she is ready to lay she will go to the dirt and check it out.. She may dig several test holes before she actually digs the hole she will lay in.. If you see her digging DO NOT DISTURB HER. If she feels like you are watching her she may abandon her hole all together and that can lead to becoming eggbound.
YOu will know she is all done laying because her hole will be completely covered back up.. she will be skinny and probably very dirty. offer her food and water her really well.. She will be very tired after all that work.
Once she is done laying the eggs, her parental role is finished. YOu can remove the eggs.
What do I put them in? What temperature? What humidity? Do I turn the eggs?
Put the eggs in a tuperware or deli style container with some small holes punched in the lid.. You can use vermiculite or hatchrite for your egg medium. Incubation is about 72-80 degrees for 7 to 10 months (thats without doing diapause) Humidity is about 85% You may have to add water to your egg medium over the course of the incubation.
Do not turn the eggs. Actually after a certain stage of development, eggs can actually die from being turned. An air pocket forms and if the egg is turned the fetus can suffocate.
Here are some of my youtube videos on the subject
Laying Bin part one and two:
http://www.youtube.com/watch?v=DOfwTpO_biI
http://www.youtube.com/watch?v=d5Spg9USTU8
And on Incubation
http://www.youtube.com/watch?v=E0Rq-EzsBuY
Also this blog by Jim at the chameleon company is excellent reference
http://www.chameleoncompany.com/Breeding.html