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Veterinarian Practice & General Health Issues Anything to do with veterinarians, health issues, pathogens, hygiene, or sanitation.

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Old 05-18-2014, 04:58 PM   #31
April Homich
I'm fully aware of how snakes are intubated. I've helped with it myself on more than one occasion. I understand that feeding a tube into the trachea can be done accidentally on dogs and cats because the trachea opening is further down the throat. Ive seen it done (though it was noticed during the initial check of the tube) and saw the aftermath from another clinic (a lung full of charcoal). As Harold is also saying, this isn't an issue in snakes. I just felt more was made out of it than needed to be. In snakes, it's not one of the risks when force feeding. There's no confusing the openings. If you start in the back of the mouth, the only place to go is the esophagus.
 
Old 05-18-2014, 06:38 PM   #32
Lupine
Quote:
Originally Posted by April Homich View Post
I'm fully aware of how snakes are intubated. I've helped with it myself on more than one occasion. I understand that feeding a tube into the trachea can be done accidentally on dogs and cats because the trachea opening is further down the throat. Ive seen it done (though it was noticed during the initial check of the tube) and saw the aftermath from another clinic (a lung full of charcoal). As Harold is also saying, this isn't an issue in snakes. I just felt more was made out of it than needed to be. In snakes, it's not one of the risks when force feeding. There's no confusing the openings. If you start in the back of the mouth, the only place to go is the esophagus.
I wasn't going to go on anymore, but I feel I need to give a (short) reply. As in my last post, if I hadn't had someone bring in a snake after tube feeding thru the trachea, I wouldn't have (OK, I shouldn't have anyway) brought it up, yes I see that I made too much of it. Sorry. I do also see that the "as well" part of my reply makes no sense as well as a few other places for confusion. I actually had a lot more there and cut a bunch out to make it not so long and rambling, and wound up leaving some extra words and other things that were a little out of place/order. I shall be much more careful when cutting/pasting, and more concise from now on. Sorry to all for the confusion and commotion.

Perhaps to get back on track- maybe the OP can give us an update?
 
Old 05-18-2014, 10:03 PM   #33
hadenglock
Quote:
Originally Posted by Lupine View Post
I don't understand this reply. You just summed up what I had said in reply to your previous statement "...bloodwork will rule out sooo many different pathogens that could be the culprit...". I am aware there are many major independent and university labs, some of which run things others don't. It sounds like you work for a vet who specializes in exotics (?) and you seem to know more about the reptile pathogen tests available than I do, so please enlighten us on what's out there, particularly with advances in treating these viral infections.
I dont know much about how they specifically run the tests, all i know is that my doctor would be pulling blood on this animal from the first visit, and if it were my animal i would leave no stone unturned. One simple way for finding viral infections is looking for inclusions in blood cells. Rotors can be used for finding elemental values in the animals blood, if you know what the "standards" are for that animal you can try comparing them to it and see if somethings off. You can find these "standard" values in texts books like Dr. Maders.
 
Old 05-19-2014, 12:51 AM   #34
Lupine
Quote:
Originally Posted by hadenglock View Post
I dont know much about how they specifically run the tests, all i know is that my doctor would be pulling blood on this animal from the first visit, and if it were my animal i would leave no stone unturned. One simple way for finding viral infections is looking for inclusions in blood cells. Rotors can be used for finding elemental values in the animals blood, if you know what the "standards" are for that animal you can try comparing them to it and see if somethings off. You can find these "standard" values in texts books like Dr. Maders.
OK, I'm going to give this another try, and make it short.

The U of Fla has developed an agglutination test for paramoxyvirus (which I didn't know about until someone posted a link to their site), however it is much like testing for Rocky Mtn Spotted Fever titers, here's a quote from UF's website regarding it:

"A hemagglutination-inhibition test (Figure 14) has been developed to determine the presence of specific antibodies to OPMV in plasma/sera of exposed snakes. Blood samples are easily obtainable by cardiac puncture. See submission of samples below...As in mammals, a positive titer is simply indicative of exposure to OPMV. Based upon a single sample, it would be impossible to make a statement about presence of virus and shedding status. If 2 samples are obtained form the same animal at a 2-4 week interval, and a rising titer can be demonstrated, this would be supportive evidence for recent OPMV infection."

It sounds to me like this would only be useful for identifying snakes in a collection that were exposed but not yet clinical, as the time needed (up to 4 weeks) is unrealistic with a sick snake. For a sick snake, histopath is still the way to go.

For IBD they(pathologists at a lab) do read blood smears looking for inclusion bodies, however, this is not considered reliable due to false negatives, according to UF "we do not know how often inclusions are seen in a peripheral blood film of a snake with IBD. It must be remembered that absence of inclusions in a blood film does not necessarily mean the snake is free of IBD." Inclusion bodies are hard to rely on for a diagnosis in any species/virus because they are not always going to be there. Again, histopath is generally relied on, at least among the few herp vets I know. But UF says they are working on a blood-based immunohistochemical staining test. Hopefully they market that soon.

Paramoxy, IBD and Adenovirus are the only viruses I know of affecting snakes (and Adenovirus is questionable in snakes IMO) that can be tested for at a lab. The point of my posts in regard to this matter, has been that there is no (as far as I am aware or can find) in-house or "simple" blood test for a snake virus. Histopath testing for IBD/Paramoxy is, IMHO, too expensive for most people to warrant running them with no basis for suspicion of a viral infection. My whole point is, I just don't think it's as simple as you think it is to identify the specific viral or bacterial pathogen in a live snake (barring fecal exam for GI parasites/infections), and certainly not thru in-house bloodwork.
 
Old 05-19-2014, 06:13 PM   #35
AbsoluteApril
Quote:
Originally Posted by Lupine View Post
You should be pushing the med into the tube until it reaches the tip before introducing the tube into the snake, then after administering, flush the tube with the liquid food if you're feeding too, or the right amount of water to push the rest of the med thru.
yes, actually I did do that (made sure the med was coming out the end of the tube before putting it in her) but I did not mention it in the vid which I should have (I was very stressed when making that). I didn't know about flushing it the rest of the way through. I believe my vet had given enough so what dose did go in was enough because I seem to remember him saying that it didn't matter what was left in the tube, but I don't recall exactly. Anyways... good points, thank you.


edit to add I found this photo of one of my boas yawning - the glottis is the part that lays along the bottom of the mouth, like a flesh tube, don't put the tube down there. XD
 
Old 05-20-2014, 01:38 AM   #36
Lupine
Quote:
Originally Posted by AbsoluteApril View Post
edit to add I found this photo of one of my boas yawning - the glottis is the part that lays along the bottom of the mouth, like a flesh tube, don't put the tube down there. XD
Yep!- don't know why I have to make things so difficult And Awesome pic!
 
Old 05-20-2014, 10:12 AM   #37
yeloowtang
Thanks everyone so much for all the info, I was away a few days so i didn't get to read all of the replies..

just finished reading

I will try to get to everything witghout leaving out info, and give an update plus the plan for the next fee days.

for the bloddwork, I honestly don't know why/what reason they had for not feelibng it was needed ?? I will ask again next time i bring her in.

I haven't force fed or tub fed yet, but I did try to feed normally a small rat pup, i was sure she was going to take it, was out, smelling it and roaming all over it.. but in the end she left it there.... soooo i tossed it out the next morning, figured maybe she wil eat it over night..

as for the tube feeding, i'm still on the fence about witch route to take.
My initial feeling was as April said, that it would be less stressfull ??

I have no problem knowing where the tube should go in, that part is easy.
It's the rest that i'm reading on, more precisely is how far in is it safe to release the food..

from what i read, the tube should go in about 3/4 of the ways down, then i should feel restriction, this means i'm at the stomach...
so at tghis point, do i releas the food or do we need to go slightly in more to inject inside the stomach, or is releasing the food at the entrance the way to do it ???

the way i would set up is, suck the amount of food needed from the tube it self, there may be no food in the seringe itself since it will be all inside the tube, this way no air would be injected and the total amount desired would go in..

unrelated to snakes, but i've been keeping salt water reef for over 20 years now, all the testing and additives are done this way, when dosing, you have to factor in the air space taken from the needle or the tip, you find yourself with an air pocket inside the seringe between the plunger and the fluides, this is normal, you all know this, but when helping out others, many don't know this and fill, tap to get the air out and then refill the ex: 2cc mark
with a small 0.5 cc seringe it may not make a big difference but with bigger ones and certain meds , one could overdose.. I know that in salt water testing, some test are thrown completly off if this is done..
so using a 36" + long tube full of food there may be none inside the seringe, but it would all be inside the tube.

thanks for all the videos, watching them all , and some i've seen before

update on the snake, well she hasn't eaten yet, but the soakings seem to help out the hydration part, her skin seems to fit her body now ( if this makes sense)
she started looking like a pug
the redness /inflamation has gone away, traces are left as if the blood dried ??? and with her last shed, it isn't visible all that much.
most of her dry scales are getting better.
along with the soakings, after 15 mins in luke war water, I add pavidone (betadine) solution to help..
the RI seems to be gone now, and the last 2 pees she had , her sent gland went back in on it's own.... that alone is a stress factor gone... i think it was related to her being dehydrated.
don't get me wrong, we aren't out of the woods yet !!! until she starts feeding and gaining weight back, i'm extremely nervous about her final outcome, at least now things are looking a little better..

looking forward to any and all info you may add, feel free to share experiences as well, I would never consider it an ijack but good info to read.

thanks you all so much again

Steph
 
Old 05-20-2014, 02:51 PM   #38
bcr229
Quote:
Originally Posted by Lupine View Post
For IBD they(pathologists at a lab) do read blood smears looking for inclusion bodies, however, this is not considered reliable due to false negatives, according to UF "we do not know how often inclusions are seen in a peripheral blood film of a snake with IBD. It must be remembered that absence of inclusions in a blood film does not necessarily mean the snake is free of IBD." Inclusion bodies are hard to rely on for a diagnosis in any species/virus because they are not always going to be there. Again, histopath is generally relied on, at least among the few herp vets I know. But UF says they are working on a blood-based immunohistochemical staining test. Hopefully they market that soon.
The molecular PCR blood test for IBD became available earlier this year. Blood samples have to be submitted by a veterinarian. The lab's web site is http://labs.vetmed.ufl.edu/sample-re...ed-infections/ if you want a test done. They suggest two tests 90 days apart to reduce the chance of false negative results. The cost is $100 for the test, plus whatever your vet would charge for the blood draw and shipping the sample to the lab.
 
Old 05-24-2014, 11:20 PM   #39
Lupine
Quote:
Originally Posted by yeloowtang View Post
unrelated to snakes, but i've been keeping salt water reef for over 20 years now, all the testing and additives are done this way, when dosing, you have to factor in the air space taken from the needle or the tip, you find yourself with an air pocket inside the seringe between the plunger and the fluides, this is normal, you all know this, but when helping out others, many don't know this and fill, tap to get the air out and then refill the ex: 2cc mark
with a small 0.5 cc seringe it may not make a big difference but with bigger ones and certain meds , one could overdose.. I know that in salt water testing, some test are thrown completly off if this is done..
so using a 36" + long tube full of food there may be none inside the seringe, but it would all be inside the tube.
Sorry it took so long to reply.

I've been a reef keeper since the 90's too, and use syringes from work instead of those plastic droppers they give you with test kits. Anyway, I want to say that all syringes are marked accounting for the amount remaining in the hub. When you remove the air bubble from the hub (or don't get one in the first place), and the syringe is drawn to, say, the 1 mL line, when you express the plunger all the way (injecting the liquid out) you will have dispensed exactly 1 mL, because the hub remains filled when the plunger is all the way in. So overdosing is not possible.

The amount still in the tube is why I advised measuring how much the tube holds, and pushing that much water thru to get all the food/med dispensed.

Still clear as mud?
 
Old 05-26-2014, 09:55 AM   #40
yeloowtang
cool , that makes complete sense, taping to replace the air pocket would only result in waisting fluides, not injecting more.

I guess from all the instructions and techs telling not to worry about the air pocket and just draw in and stop at the Ml you need had me thinking this from habbit.when having to deal with ivermectin it's important to be carefull..

especially (in reef related) you probably heard or used the anna calcium checker
this little gem of technology, you had to replace the cheap harware supplied with medical ones, otherwise results were off by a mile.. but again , unrelated hehehe

she had another shed on the week end , she;'s now looking perfect, no more redness or dried up ugly scales...
now if she can eat, since she hasn't lost anymore weight, i'm going to keep trying normal feeding for a while before streesing her with either force feeding or tub feeding..
she was out cruising last night.. will try to feed her tonight and hope she snags it..
 

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