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Sonograms and forced eversion of the hemipenes with a saline injection while the animal is "under" are the most accurate... Sperm plugs are an easy identifier of males, but don't guarantee females... The head width is sketchy, I know I can't accurately sex them visually (I know one person who can though, so I guess it's possible, just... really, really really difficult)... Watching copulation, but you get females who'll beat the crap out of other females and males who beat the crap out of anything.
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