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How long after thawed is rat still safe to feed boa?

Hard to say for sure. Best way to tell is the sniff test. If there even a hint of "dead" smell, I wouldn't feed it. Generally, I'd say anything over 12 hours is no good for a snake.
 
If you put it in the enclosure at night and it's still there in the morning dump it (or offer to another snake if that's a good alternative) or about 8 hours for a daytime feeder is my limit. If the snake hasn't eaten it by then it's not going to; wait a week and try again.
Warming up the item and teasing the snake a bit to see if it's interested may help, though some snakes are simply best left alone with the item.
 
It's pretty disgusting but I did the overnight thing with one of my picky male ball pythons about 8:00 pm. When I checked him at 7 a.m. the following morning he was in the process of swallowing it. He suffered no ill effects.
 
Leaving a thawed prey item overnight is fine. Some will eat it right away, others will wait until it is dark with no outside movement. Either way, toss anything left in the morning.

Be careful of re-feeding as well: if a snake has passed on an item, and it later turns out to be due to illness, a refeed can spread that illness very quickly.
 
I have just had a relevant experience - it's very hot here right now, and my snakes have regurgitated their food animals more than once. I think I have been leaving them out too long between thawing and feeding.

So part of the answer to this might be "depends on the temperature"
 
I have just had a relevant experience - it's very hot here right now, and my snakes have regurgitated their food animals more than once. I think I have been leaving them out too long between thawing and feeding.

So part of the answer to this might be "depends on the temperature"
Have you tried thawing the feeders in the refrigerator for a day and then just heating them up as needed with warm water right before feeding? That way they don't sit at room/warm temp for long before you feed.
 
I think that's a good idea.

We're having daytime temps of 34oC (that's about 93oF for you lot :>poke2<:), so I think they are going off in the hour or so it normally takes to thaw them at room temp.
 
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