I've seen it with small mammals (rats/mice) and day old chicks - don't like it. I haven't tried it with reptiles because of that, so I don't speak from personal knowledge there, but I extrapolate that as they have a slower metabolism, it would go on longer for them... have you used it? Do you have personal observations?
By the way I think it's great to have this discussion in such a calm manner. I've seen threads on this before where it gets very heated, and no one learns when accusations get thrown around, so congratulations on giving this a forum without it turning into a S**T fight.
People need to be able to ask these questions without getting attacked
Ouch. And they weren't unconscious while gasping? If they were unconscious, it wouldn't be a problem, but if they were, yeah, that's a no-go. Then again, it's really hard to tell when something is conscious. :-/
I did some more quick research on CO2, and learned a few things. Like for small mammals, one needs specific concentrations to cause rapid loss of consciousness, which I hadn't thought of.
In terms of reptiles, and it seems like some say it's okay "for certain species" and some say it's bad. (I have yet to find out what these "certain species" are. Grr.) It does make sense, though, that for reptiles, it would be a bad idea. Their slow metabolisms and breath-holding ability would make it take a long time to die, if at all. Again, a "long time" doesn't mean "painful," but it does mean one would need quite a bit of CO2.
Either way, I'm not liking how CO2 is looking, especially in terms of reptiles. I did CO2 a rat once with dry ice in a 10-gallon tank. Because of the condensation, I was unable to see how the rat reacted, but there was no obvious (audible) thrashing or anything of the sort, and it took mere minutes for the rat to pass away. Still, that's one rat, and I wasn't able to see it. I'd have to do more research before doing it again, obviously, especially with your first-hand experience.
Me too, in terms of discussing in a calm manner. :-] I'm grateful for that, most definitely. You're absolutely right: it's hard to learn anything in a flame war. It's sad when people are afraid to ask questions for fear of being yelled at or insulted. I can't take all the credit for that, though; responders have to be calm, too, so thank y'all for being civil as well.
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For freezing reptiles, in general, I have a few more resources.
Another one from a vet (posted on Melissa Kaplan's reptile pages):
http://www.anapsid.org/euth.html
A UK report:
http://www.rabiesblueprint.com/IMG/pdf/Link72_Euthanasia_EC_Part2.pdf (includes that CO2 is not acceptable for reptiles).
And an additional paper (
http://www.thefrilleddragon.com/content/129-Euthanasia-of-Reptiles) that contains the following (emphasis mine):
"Because ectotherms become progressively inactive with lowering of body temperature, it has been assumed in the past that cooling them prior to euthanasia would not only facilitate handling, but would anaesthetise them. Reptiles immobilised in this way were then placed in a domestic freezer and killed by subsequent freezing. This method of euthanasia is not acceptable in the light of modern knowledge. Although decreasing temperature decreases metabolic rate and decreases response to stimuli, it is not known how it affects pain perception impulses in various nerve bundles are affected differently by temperature and some authors feel that pain neurons are less cold affected than motor neurons. Most published guidelines on euthanasia now include statements to the effect that
although cooling ectotherms produces torpor, it does not reduce their ability to feel pain2,3,5, and indeed it may prolong suffering since a lowered metabolic rate would delay the onset of unconsciousness due to hypoxia. Humans dying from hypothermia are said to experience numbness, even euphoria; but this is believed to be due to endorphin production by the mammalian, endothermic brain; there seems to be no evidence for this in reptiles.
A conscious reptile placed in a freezer would experience a very painful death. Destruction of its skin and eyes due to ice crystal formation within the cells, causing intense pain, would occur long before the brain had become chilled enough to cause loss of consciousness, especially in the case of a large reptile such as an iguana, whose brain would take a considerable time to chill. Even inducing hypothermia for facilitating the handling of reptiles is now considered inappropriate and inhumane by many authorities5, and
freezing in a domestic freezer should not be accepted as a method of euthanasia for any unanaesthetised animal of any species.
"The Euthanasia Working Party (EWP) report published in Laboratory Animals (International Journal of Laboratory Animal Science and Welfare)3 on the recommendations for euthanasia of experimental animals states that
hypothermia ' ..will make the animal torpid but will not raise the pain threshold. The formation of ice crystals within the body tissues is likely to be extremely painful. Hypothermia is not acceptable for euthanasia.' This is a comment typical of many found throughout the literature. The only mention of refrigeration in the Home Office Code of Practice for the Humane Killing of Animals4 is for small foetal, larval and embryonic forms of animal and even so, reptile eggs and embryos are specifically
excluded from this method because of their resistance to chilling and hypoxia. In the laboratory situation dropping into liquid nitrogen at minus 1960C – a very extreme form of freezing, far removed from a domestic freezer may be acceptable for animals of less than 40g bodyweight (i.e. less than 1cm in diameter) as liquid nitrogen would freeze an entire body of that size instantaneously.10"