I just wanted to let you all know that I have been in contact with Dr. Jacobson about testing... As soon as I find out what he needs me to do as far as sending samples from his intern, Miss Rita Chang, I will be sending them.
This is the email that he wrote me after I asked him a few questions. Yes, I did respond and tell him that I had the BCL die in Nov. 2009 with inclusions in the report. I had wrote him about the BCL when he died, but over a year ago is a long time to remember with all the work he is doing.
I also plan to give a good sized donation to him around tax time.
Here is the email where he responded to my questions.
from Jacobson,Elliott R <
[email protected]>
to Ashley Caspillo <
[email protected]>
cc "Chang,Li-Wen" <
[email protected]>
date Mon, Jan 10, 2011 at 9:04 PM
subject RE: IBD Blood and Tissue tests
mailed-by ufl.edu
hide details 9:04 PM (1 hour ago)
Ashley:
Here are answers to your questions:
1. I was wondering how accurate the blood test is vs. the liver sample? We do not know since we do not have a large enough data-base of blood samples and liver samples from the same animal. Based on a small number of boas, all that had inclusions in liver, kidney, pancreas, or esophageal tonsils, also had inclusions in peripheral blood cells. My graduate student Rita Chang (
[email protected]) has developed an immunohistochemical staining test that is based on an antibody that recognizes the protein in the IBD inclusions. If inclusions are present, they will be stained. We recommend that this testing be first performed since it is less expensive than having a biopsy collected and submitted for staining. Staining of a liver sample would be the same as blood-staining.
2. I was also wondering how likely it is to have IBD floating around since I have not had any more IBD related deaths and no other deaths since May 2010, which was my burm showing no inclusions, that 30+ boas and pythons would not show any signs of IBD or die from IBD? Has IBD been diagnosed in any snake in your collection. It appears that your Burmese died of something else. We do not know how long snakes may be infected without showing clinical signs. Those snakes that do not show signs are called subclinical cases. They may remain subclinical from weeks to months to years. It is possible some may have inclusions at a low level for a very long period of time. As we receive more and more samples, especially from what appear to be normal animals, we will be able to get a better idea of the correlation. This is a long-term project. Luckily we received a small grant from an animal foundation to do this research. This is Rita Chang’s PhD research project. New tests will be evaluated by Rita and will be evaluated over the next few months.
3. I still want to get blood and/or liver testing done and was wondering how much the liver testing would be? Liver testing would be the same as blood testing. But some snakes may have inclusions in kidney and pancreas and not liver.
4. Also, about how long do the results take to get back? Rita Chang can answer this question. The slides are stained in our medical school pathology laboratory and are worked up as time permits in their staining schedule.
Attached is a paper I may not have sent you. It helps explain the immunohistochemical stain that Rita has developed. Soon we will have a webpage devoted to IBD testing. The big question is: What to do with positive snakes? This will depend on whether they are ill or not. Those that are ill should be euthanized. Those that are healthy should be separated from ill snakes and followed over time. It may take more than one blood test to confirm if a snake is positive or negative. At this point we recommend 2 blood samples 90 days apart. We have selected 90 days since this is the length of quarantine we recommend for new animals coming into an established collection. Mites have been associated with outbreaks so be aware of this.
I hope this answers your questions and thanks for considering a donation.
Sincerely,
Elliott Jacobson
Professor of Zoological Medicine
and he attached the paper that he had also sent to Courtney